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Ubio Biotechnology Systems Pvt Ltd
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Image Search Results
Journal: Cell Death Discovery
Article Title: Pregnane X receptor protects against age-related bone loss in males via PI3K/Akt-mediated inhibition of apoptosis
doi: 10.1038/s41420-025-02797-y
Figure Lengend Snippet: A Schematic diagram of experimental design. B ELISA for inflammation factors Tnfα, Il1β, and Il6 in the conditional medium collected from Nr1i2 knockdown primary BMSCs or siNC as control. C ELISA for ROS clearance-related enzyme T-SOD, GSH-PX and ROS damage biomarker MDA in Nr1i2 knockdown primary BMSCs or siNC as control. D Flow cytometry analysis of intracellular ROS level by using the fluorescent dye DCFDA. Western blot for PI3K/Akt pathway- ( E ) and apoptosis- ( F ) related proteins in primary BMSCs of different groups. Data were means ± s.e.m. n = 3 independent repeats for in vitro experiments. ** p < 0.01, *** p < 0.001 by t-test.
Article Snippet: The Il1β, Il6, and Tnfα level in the conditional medium, intracellular level of T-SOD, GSH-PX, MDA, and ROS were determined by using the Mouse IL-1β ELISA Kit (HJ177, Epizyme Biotech, Shanghai, China),
Techniques: Enzyme-linked Immunosorbent Assay, Knockdown, Control, Biomarker Discovery, Flow Cytometry, Western Blot, In Vitro